Comparative karyotype analysis of two Iranian cyprinids, Alburnoides bipunctatus and Alburnus filippii (Cypriniformes, Cyprinidae)

Document Type : Research articles

Authors

Sajad Nazari ¹

M. Pourkazemi ²

J. I REBELO PORTO ³

¹ Department of Fisheries, Faculty of Natural Resources, University of Guilan, P. O. Box 1144, Iran

² International Sturgeon Research Institute, P. O. Box 41635-3464 Rasht, Iran

³ Coordenação de Pesquisa em Biologia Aquática, Instituto Nacional de Pesquisas da Amazônia,Manaus, AM, Brazil

10.22067/ijab.v5i2.3335

Abstract

This study provides new data on chromosomal characteristics of two cyprinid fish species found in Iran, Alburnoides bipunctatus and Alburnus filippii. The chromosomes of these species were compared using Giemsa staining and silver-staining (Ag) techniques to describe the karyotypes. Both species possess 2n = 50 chromosomes with no detected sex heteromorphism. The karyotypes were distinct, composed of 7 pairs of metacentric, 10 pairs of submetacentric, and 8 pairs of subtelo-acrocentric chromosomes in A. bipunctatus and 6 pairs of metacentric, 9 pairs of submetacentric, 4 pairs of subtelocentric, and 6 pairs of acrocentric chromosomes in A. filippi. Both species possessed a single pair of Ag-
stained nucleolar organizer region (NOR)-bearing chromosomes, but the species differed in its location and chromosomal type. In A. bipunctatus, Ag-NORs were located on the telomeres of two submetacentric chromosomes, and in A. filippii, on the telomeres of the short arm of a subtelocentric chromosome pair. The NOR patterns were nearly identical
with those found in other representatives of Eurasian cyprinids and support the phylogenetic relationships proposed for species in the Leuciscinae. The results of cytogenetic studies of related species, mainly in the Leuciscinae, are compared and
discussed.

Keywords

Cypriniiformes

Alburnoides bipunctatus

Alburnus filippii, Fish cytogenetics, NOR, Cytotaxonomy

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