Application of microsatellite markers to determine populations of the Persian sturgeon (Acipenser persicus) in the South Caspian Sea

Document Type : Research articles



The population genetic structure of the Persian sturgeon (Acipenser persicus) in the
Sefidrud and Gorganrud River watersheds was analyzed based on the characterization of
microsatellite markers. One hundred fin clip samples of Persian sturgeon from the two
regions were collected. Four microsatellite loci (Ls68, Spl168, Spl173, and Afu68) were
analyzed for molecular characterization, resulting in polymorphic patterns. DNA bands
were analyzed using Biocapt and GenAlex software packages. In total, 109 alleles were
observed. The maximum number of alleles (17) was found in locus Spl168 in sturgeon
from the Sefidrud watershed and the minimum number of alleles (10) in locus Ls68 in
sturgeon from the Gorganrud watershed. No significant differences between samples of
the two regions, either in average number of alleles per locus or in heterozygosities, were
observed (P>0.05). However, based on AMOVA, the calculated Fst (0.07) and Rst (0.17)
between the two regions were significantly different (P