Molecular genetics and divergence time study of the cone snail species in the Persian Gulf

Document Type : Research articles


1 Khorramshahr University of Marine Sciences and Technology

2 Khorramshahr University of Marine Science and Technology

3 Hormozgan University


Marine gastropod genus' Conus diversified rapidly during the Miocene includes the most species-rich modern marine genus. The aim of this study is an expanded molecular phylogenetic analysis and divergence time of two dominant cone snail species from the Persian Gulf. The mitochondrial cytochrome oxidase subunit I sequence data of Conus coronatus and Conus frigidus species from the Persian Gulf were used to compare with the other Conus species in Viroconus and Virgoconus clades from the different regions. Moreover, divergence time of the Persian Gulf clusters was estimated from the substitution rate of the genome. Results showed, low differences between the Conus species of the Persian Gulf and Indo-Pacific, and divergence time of the Persian Gulf Conus species was about 2 million years ago. So, the Persian Gulf Conus species originated from the Indo-Pacific parallel to the geological events and after the Ice Age. Then, these species were transferred through the Indian Ocean to the Persian Gulf. These findings give use a knowledge of the origin and the evolution of these species in the Persian Gulf.


Akbari, T., Masoudian, S. A. 2009. Regionalization of temperature regions of Iran. Geography Environmental Planning Seasonal Journal 33 (1), 59–74 (in Persian, abstract available in English).
Al-Khayat, J. A. 2008. Molluscs of the state of Qatar. Qatar Biodiversity Newsletter 2, 1-5.
Bandyopadhyay, P.K., Stevenson, B.J., Cady, M.T., Olivera, B.M., Wolstenholme, D.R. 2006. Complete mitochondrial DNA sequence of a Conoidean gastropod, Lophiotoma (Xenuroturris) cerithiformis: gene order and gastropod phylogeny. Toxicon 48, 29–43.
Bandyopadhyay, P.K., Stevenson, B.J., Ownby, J.P., Cady, M.T., Watkins, M., Olivera, B.M. 2008. The mitochondrial genome of Conus textile, coxI-coxII intergenic sequences and conoidean evolution. Molecular Phylogeny and Evolution 46, 215–223.
Biass, D., Violette, A., Hulo, N., Lisacek, F. and Favreau, P. 2015. Uncovering intense protein diversification in a cone snail venom gland using an integrative venomics approach. Journal of Proteome Research 14, 628-638.
Bouchet, P., Kantor, Y., Sysoev, A., Puillandre, N. 2011. A new operational classification of the Conoidea (Gastropoda). Journal of Moluscan Study 77, 273–308.
Brauer, A., Kurz, A., Stockwell, T., Baden-Tillson, H., Heidler, J., Wittig, I., Kauferstein, S., Mebs, D., Stocklin, R., Remm, M. 2012. The Mitochondrial Genome of the Venomous Cone Snail Conus consors. Plos one 7 (12), 1-10.
Craik, D. J., Fairlie, D. P., Liras, S. and Price, D. 2013. The future of peptide-based drugs. Chemical Bioligy and Drug Design 81 (1), 136-147.
Cunha, R. L., Tenorio, M. J., Afonso, C., Castilho, R., Zardoya, R., 2008. Replaying the tape: recurring biogeographical patterns in Cape Verde Conus after 12 million years. Molecular Ecology 17, 885–901.
Duda, T. F., Kohn, A. J., Palumbi S. R. 2001. Origins of diverse feeding ecologies within Conus, a genus of venomous marine gastropods. Biological Journal of the Linnean Society 73, 391–409.
Duda, T. F., Kohn, A. J. 2005. Species-level phylogeography and evolutionary history of the hyperdiverse marine gastropod genus Conus. Molecular Phylogeny and Evolution 34, 257–272.
Duda, T. F., Palumbi, S. R. 1999. Molecular genetics of ecological diversification: duplication and rapid evolution of toxin genes of the venomous gastropod Conus. Proceed National Academy Science 96, 6820–6823.
Duda, T. F. 2008. Differentiation of venoms of predatory marine gastropods: divergence of orthologous toxin genes of closely related Conus species with different dietary specializations. Journal of Molecular Evoluation 67, 315-321.
Dutertre, S., Jin, A. H., Kaas, Q., Jones, A., Alewood, P. F., Lewis, R. J. 2013. Deep venomics reveals the mechanism for expanded peptide diversity in cone snail venom. Molecular and Cell Proteome 12, 312–329.
Espino, S. L., Kohn, A. J., Villanueva, J. A., Heralde, F. M., Corneli, P., Concepcion, G. P., Olivera, B. M., Santos, A. D. 2008. Feeding behavior, phylogeny, and toxinology of Conus furvus Reeve, 1843 (Gastropoda: Neogastropoda: Conidae). Nautilus 122, 143–150.
Folmer, O., Black, M., Hoeh, W., Lutz, R., Vrijenhoek, R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3, 294–299.
Hu, H., Bandyopadhyay, P. K., Olivera, B. M., Yandell, M. 2011. Characterization of the Conus bullatus genome and its venom-duct transcriptome. BMC Genomics 60, 1471-2164.
Kaas, Q., Westermann, J., Craik, D. J. 2010. Conopeptide characterization and classifications: An analysis using ConoServer. Toxicon, 55(8), 1491-1509.
Kraus, N. J., Watkins, M., Bandyopadhyay, P. K., Seger, J., Olivera, B. M., Corneli, P. 2012. A very short, functionally constrained sequence diagnoses cone snails in several Conasprella clades. Molecular Phyogenyl Evoluation 65, 335–338.
Lewis, R. J., Dutertre, S., Vetter, I., Christie, M. J. 2012. Conus Venom Peptide Pharmacology. Pharmacol Review 64, 259–298
Lorenz, F., Puillandre, N. 2015. Conus hughmorrisoni, a new species of cone snail from New Ireland, Papua New Guinea (Gastropoda: Conidae). European Journal of Taxonomy 129, 1–15.
Nam, H.H., Corneli, P.S., Watkins, M., Olivera, B., Bandyopadhyay, P. 2009. Multiple genes elucidate the evolution of venomous snail-hunting Conus species. Molecular Phylogeny and Evoluation 53, 645–652.
Palumbi, S.R. 1996. PCR and molecular systematics. In Molecular Systematics, 2 edition, D. Hillis, C. Moritz, A. and Mable B. Eds. Sinauer Press, 205-247 pp.
Pereira, C.M., Rosado, J., Seabra, S.G., Pina-Martins, F., Paulo, O.S., Fonseca, P.J. 2010. Conus pennaceus: a phylogenetic analysis of the Mozambican molluscan complex. African Journal of Marine Science 32, 591–599.
Puillandre, N., Samadi, S., Boisselier, M.C., Sysoev, A.V., Kantor, Y.I., Cruaud, C., Couloux, A., Bouchet, P. 2008. Starting to unravel the toxoglossan knot: molecular phylogeny of the ‘‘turrids’’ (Neogastropoda: Conoidea). Molecular Phylogeny and Evoluation 47, 1122–1134.
Puillandre, N., Bouchet, P., Duda, T.F., Kauferstein, S., Kohn, A., Olivera, B. M., Watkins, M., Meyer, C. 2014. Molecular phylogeny and evolution of the cone snails, Molecular Phylogeny and Evoluation 78, 290-303.
Rambaut, A., Drummond, A.J. 2007. Tracer v1.4. <>.
Rodreguez, A. M., Dutertre, S., Lewis, R. J., Mari, F. 2015. Intraspecific variation in Conus purpurascens injected venom using LC/MALDI-TOF-MS and LC-ESI-Triple TOF-MS. Analytical and Bioanalytical Chemistry 407, 6105-6116.
Ronquist, F., Huelsenbeck, J. P. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572–1574 pp.
Stamatakis, A. 2006. RAxML-VI-HPC: maximum likelihoodbased phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22, 2688–2690.
Tamura, K., Stecher G., Peterson, D., Filipski, A.m Kumar, S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0.
Violette, A., Biass, D., Dutertre, S., Koua, D., Piquemal, D., Pierrat, F., Stöcklin, R. Favreau, P. 2012. Large-scale discovery of conopeptides and conoproteins in the injectable venom of a fish-hunting cone snail using a combined proteomic and transcriptomic approach. Journal of Proteomics 75 (17), 5215– 5225.
WoRMS. 2014. World Register of Marine Species. Available from http://