Comparison of the body shape of Aphanius vladykovi populations (Teleostei: Aphaniidae) using geometric morphometric method

Document Type : Research articles

Authors

1 Department of Animal Sciences, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran

2 Department of Animal Sciences, Faculty of Basic Sciences, Shahrekord University, Shahreko

3 Department of Biodiversity and Ecosystem management, Environmental Sciences Research Cent

4 Department of Fisheries and Environmental sciences, Faculty of Natural Resources and Eart

Abstract

The main purpose of this study was to investigate the morphological changes and body shape variations of the Zagros Tooth-carp Aphanius vladykovi populations in six water bodies located in Chaharmahal and Bakhtiari province, Iran. For this purpose, 240 specimens were collected from the Choghakhor and Gandoman wetlands, the Shalamzar, Balagholi, and Brovi springs, and the Beheshtabad River. Twelve landmark points were digitized on the right side of the fish. Statistical analyses including Principal Component Analysis (PCA), Discriminant Function Analysis (DFA), and Cluster Analysis (CA) were performed using TPS package, SYSTAT 9, and PAST Software. Discriminant function analysis revealed that female fish in different localities show more pronounced body shape differences compared to males from different localities (p <0.001). In addition, in clustering analysis performed among males and among females, two clusters were resolved, which were fairly similar for males and females. These differences might be attributed to the morphological adaptation of the Zagros Tooth-carp to their habitats but a clear inference on the observed morphological divergence will be possible with more genetic, ecological, and experimental evidence.

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Abedi, M., Egdari, S., Ghafari Farsani, H., Shahbazi Naserabad, S., Benam, S., 2016. Morphological flexibility of Pearl Zagros fish (Alburnus zagrosensis, Code, 2009) at Choghakhor wetland, Chshmeh ALi and Hamzeh ALi areas, Charmahal Bakhtiari Province, Iran. The Forth Iranian Conference of Ichthyology, Ferdowsi University of Mashhad, 20-21 July 2016.
Abolhasani Koupaie, F., Hashemzadeh Segherloo, I., Heidarnejad, M.S., Tabatabaei, S.N., 2020. DNA barcoding of Aphanius vladykovi from different habitats in Chaharmahal va Bakhtiari Province, Iran. International Journal of Aquatic Biology 8(1), 66-72.
Bibak, M., Sattari, M., Heidari, A., 2016. Morphological variation in two populations of Aphanius dispar (Pisces: Cyprinodontidae) from two sulfur springs in Bushehr, south of Iran. Caspian Journal of Applied Sciences Research 5(4), 36-43.
Burnett, K.G., Bain, L.J., Baldwin, W.S., Callard, G.V., Cohen, S., Di Giulio, R.T., Evans, D.H., Gómez- Chiarri, M., Hahn, M.E., Hoover, C.A., Karchner, S.I., Katoh, F., MacLatchy, D.L., Marshall, W.S., Meyer, J.N., Nacci, D.E., Oleksiak, M.F., Rees, B.B., Singer, T.D., Stegeman, J.J., Towle, D.W., Van Veld, P.A., Vogelbein, W.K., Whitehead, A., Winn, R.N., Crawford, D.L., 2007. Fundulus as the premier teleost model in environmental biology: Opportunities for new insights using genomics. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics 2,257–286.
Cavraro, F., Malavasi, S., Torricelli, P., Gkenas, C., Liousia, V., Leonardos, I., Kappas, I., Abatzopoulos, T.J., Triantafyllidis, A., 2017. Genetic structure of the South European toothcarp Aphanius fasciatus (Actinopterygii: Cyprinodontidae) populations in the Mediterranean basin with a focus on the Venice Lagoon. The European Zoological Journal 84, 153–166.
Coad, B.W., 2017. Freshwater Fishes of Iran. www.braincoad. Com (accessed:  20 June 2017).
Eagderi, S., Esmaeilzadegak, E., Pirbeigi, A., 2014. Morphological responses of Capoeta gracilis and Alburnoides eichwaldii populations (Cyprinidae) fragmented due to Tarik Dam (Sefidrud River Caspian Sea basin, Iran). Iranian Journal Ichthyology 1, 114–120.
Esmaeili, H.R., Asrar, T., Gholamifard, A., 2018. Cyprinodontid fishes of the world: an updated list of taxonomy, distribution and conservation status (Teleostei: Cyprinodontoidea). Iranian Journal of Ichthyology 5, 1-29.
Hallerman, E.M. 2003. Population genetics: principles and practices for fisheries scientists. American Fisheries Society, Bethesda, MD, 458 pp.
Hashemzadeh Segherloo, I., Farahmand, H., Abdoli, A., Bernatchez, L., Primmer, C.R., Swatdipong, A., Karami, M., Khalili, B., 2012. Phylogenetic status of brown trout Salmo trutta populations in five rivers from the southern Caspian Sea and two inland lake basins, Iran: a morphogenetic approach. Journal of fish biology 81(5), 1479-1500.
Hashemzadeh Segherloo, I., Normandeau, E., Benestan, L., Rougeux, C., Coté, G., Moore, J.S., Ghaedrahmati, N., Abdoli, A., Bernatchez, L., 2018. Genetic and morphological support for possible sympatric origin of fish from subterranean habitats. Scientific reports 8(1), 1-13.
Hossaini, S., Shabani, A., REZAEI, H., 2013. The comparison of genetic structure of Zagros tooth-carp (Aphanius vladykovi) in Gandoman and Shalamzar wetland in the Chaharmahall-O-Bakhtiari province by using microsatellite markers. Journal of Animal Biology 5(4).
Hossaini, S., Shabani, A., REZAEI, H., 2016. Genetic diversity of zagros zebrafish (Aphanius vladykovi) in Madar-o-dokhtar spring and Chehelgazi spring in the Chaharmahall-o-Bakhtiari Province by using microsatellite markers. Journal Novin Genetics 11(1), 91-99.
Khosravi, A., Golmakan, M.Sh., Teimori, A., 2017. Variations in fish body and scale shape among Aphanius dispar (Cyprinodontidae) populations: insights from a geometric morphometric analysis. Caspian Journal Environment Sciences 15, 113-123.
Kroll, W., 1984. Morphological and behavioral embryology and spontaneous diapause in the African Killifish, Aphyosemion gardneri. Environmental Biology of Fishes 11, 21-28.
Langerhans, R.B., 2008. Predictability of phenotypic differentiation across flow regimes in fishes. Integrative and Comparative Biology 48(6), 750-768.
Pazooki, J., Sheidai, M., Korani, M.M., 2008. A systematic and ecological study of Aphanius vladykovi Coad, 1988 (Actinopterygii: Cyprinodontidae) in Iran. Zoology in the Middle East 43(1), 85-89.
Santos, A.B.I., Camilo, F.L., Albieri, R.J., Araujo, F.G., 2011. Morphological patterns of five fish species (four characiforms, one perciform) in relation to feeding habits in a tropical reservoir in south-eastern Brazil. Journal of Applied Ichthyology27, 1360-1364.
Sfakianakis, D.G., Leris, I., Laggis, A., Kentouri, M., 2011. The effect of rearing temperature on body shape and meristic characters in zebrafish (Danio rerio) juveniles. Environmental Biology of Fishes 92, 197-205.
Tabatabei, S.N., Eagderi, S., Hashemzadeh Segherloo, I., Abdoli, A., 2014. Geometric and morphometric analysis of fish scales to identity genera, species and populations case study: the Cyprinid family. Taxonomy and Biosystematics Journal 5, 1-8.
Teimori, A., Esmaeili, H. R., Erpenbeck, D., Reichenbacher, B., 2014. A new and unique species of the genus Aphanius Nardo, 1827 (Teleostei: Cyprinodontidae) from Southern Iran: A caseof regressive evolution.  Zoologischer Anzeiger - A Journal of Comparative Zoology 253, 327–337.
Teimori, A., Esmaeili, H.R., Hamidan, N. and Reichenbacher, B., 2018. Systematics and historical biogeography of the Aphanius dispar species group (Teleostei: Aphaniidae) and description of a new species from Southern Iran. Journal of Zoological Systematics and Evolutionary Research 56(4), 579-598.
Turan, C., 2008. Molecular systematics of the Capoeta (Cypriniformes: Cyprinidae) species complex inferred from mitochondrial 16S rDNA sequence data. Acta Zoologica Cracoviensia-Series A: Vertebrata 51(1-2), 1-14.
Yamamotoa, S., Maekawa, K., Tamate, T., Koizumi, I., Hasegawa, K., Kubota, H., 2006. Genetic evaluation of translocation in artificially isolated population of white spotted charr (Salvelinus leucomaenis). Fisheries Research 78, 352-358.
Zelditch, M., 2004.Geometric morphometrics for biologists: a primer. Academic Press, New York.