Genetic analysis of spring and autumn races of Caspian Sea kutum (Rutilus frisii kutum) using microsatellite markers

Document Type : Research articles



The kutum (Rutilus frisii kutum) is one of the economically valuable species of Caspian
Sea. Two different races of this fish exist in the Caspian Sea, the spring race and the
autumn race. The aims of this study were to analysis the population genetic structure and
genetic diversity among and between populations of these races based on microsatellite
markers. For this purpose, 100 samples of adult kutum from two regions of Caspian Sea
(Anzali Wetland and Shiroud River) were collected. DNA was extracted and 31 pair
microsatellite primers were used for PCR and 10 made polymorphic patterns. In this
study 191 alleles were observed totally. The maximum numbers of alleles (18) were found
in two loci (Ca1 and Ca3) and the minimum number of alleles (2) was found in MFW1
locus. The differences between both races were not statistically significant (P>0.05),
neither for average number of alleles per locus nor for observed heterozygosities. The
calculated Fst and Rst between two races was 0.056 and 0.15 which shows that the
genetic difference was significant (P< 0.01). Spring race in MFW1 locus and autumn race
in Lid1 locus were at Hardy-Weinberg equation but not in other loci in both races. The
genetic distance was 0.407 which indicating the greatest genetic distance between the two
studied races. The data generated in this study showed that the spring and autumn races
of kutum in two region of southern part of Caspian Sea are two independent populations.